Gene name |
SPBC4B4.04 |
Gene ID |
36/B10 |
Gene synonyms/obsolete |
|
Gene product |
translation initiation
factor eIF2A |
Entry clone |
Cloned |
ORF length (unspliced) |
1769 |
ORF length (spliced) |
1731 |
Entry clone length |
1769 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
811A:G / 872A:T /
1208T:C / 1516C:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC4B4.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTCAAAAAAGCCAGTT |
Rev primer name |
SPBC4B4.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGCATCCGGTGTCCATCCA |
Amino acid length |
576 |
Molecular weight |
63.4 |
Isoelectric point (calc.) |
9.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica;
DeltaVision |