Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC2H8.02
Gene ID 36/A09
Gene synonyms/obsolete
Gene product MFS inorganic phosphate transporter
Entry clone Cloned
ORF length (unspliced) 1752
ORF length (spliced)
Entry clone length 1752
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC2H8.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGCTTCAAGTTGAATCC
Rev primer name SPCC2H8.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAATGCCATGTTTCCACCAG
Amino acid length 583
Molecular weight 64
Isoelectric point (calc.) 8.2
Signal SEQ
No. of transmembrane domain 11
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFAFRVMLGI/LAGCVATLII
Localization (YFP) ER; Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 3 images) See all images

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