Gene name |
SPBP4H10.07 |
Gene ID |
36/A08 |
Gene synonyms/obsolete |
|
Gene product |
zinc finger protein;
zf-C3HC4 type (RING finger); ubiquitin ligase (E3); similar to
Sp SPCC4G3.12C (paralog); no apparentSc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1752 |
ORF length (spliced) |
|
Entry clone length |
1752 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
171A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBP4H10.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGCAAGGTGAATCCAT |
Rev primer name |
SPBP4H10.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGGCTTGGGAGAGGACGGA |
Amino acid length |
583 |
Molecular weight |
64.3 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent signal
|
Comments for localization |
vacuole and
mitochondrion? |
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica,
Confocal |