Gene name |
SPBC17D1.06 |
Gene ID |
35/H02 |
Gene synonyms/obsolete |
|
Gene product |
DEAD/DEAH box
helicase; ATP-dependent; RNA helicase |
Entry clone |
Cloned |
ORF length (unspliced) |
1737 |
ORF length (spliced) |
|
Entry clone length |
1737 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
125T:C / 1691T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC17D1.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAATTTTGTTTTAGGAT |
Rev primer name |
SPCC17D1.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTCAAAAACAATCTTAGTA |
Amino acid length |
578 |
Molecular weight |
63.9 |
Isoelectric point (calc.) |
9.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
95/68/69 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleolus>>nucleus |
Microscope used for
observation |
DeltaVision |