Gene name |
SPBC31F10.10c |
Gene ID |
35/F08 |
Gene synonyms/obsolete |
|
Gene product |
zinc finger protein;
zf-MYND type; involved in cell polarity (regulation) |
Entry clone |
Cloned |
ORF length (unspliced) |
1725 |
ORF length (spliced) |
|
Entry clone length |
1725 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC31F10.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGGGAATCGAACGTTAG |
Rev primer name |
SPBC31F10.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAATCTGCTATCACTCTGA |
Amino acid length |
574 |
Molecular weight |
64.1 |
Isoelectric point (calc.) |
9.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
SPB; periphery at site
of septum formation; nucleus>cytosol; nuclear dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |