Gene name |
SPBC19F8.06c |
Gene ID |
35/F07 |
Gene synonyms/obsolete |
meu22 |
Gene product |
meiotic expression
upregulated; amino acid permease family; similar to Sp
SPAP7G5.06 and SPAC869.11 and SPBC359.01 and SPBC359.01 and
SPBPB2B2.01 and isp5 |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1725 |
ORF length (spliced) |
|
Entry clone length |
1725 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC19F8.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTCTCCAAATTTTACGA |
Rev primer name |
SPBC19F8.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAACGCATTTTTCTCTACA |
Amino acid length |
574 |
Molecular weight |
62.6 |
Isoelectric point (calc.) |
8.5 |
Signal SEQ |
|
No. of transmembrane domain |
12 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSFAVTVPLEL |
Localization (YFP) |
periphery, especially
at cell tip and site of septum formation; cytoplasmic dots;
Golgi? |
Comments for localization |
Golgi? |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |