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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC19G7.09
Gene ID 35/E03
Gene synonyms/obsolete ulp1
Gene product Ulp1 protease family; peptidase family C48; cysteine protease; Pmt3 (SUMO)-specific protease; involved in protein deubiquitination and protein desumoylation; non-essential; involved in nuclear morphology maintenance
Entry clone Cloned
ORF length (unspliced) 1707
ORF length (spliced)
Entry clone length 1707
No. of intron 0
Sequence status Finished
Sequence results 266A:G
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC19G7.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATAGGAAAACGCAATGC
Rev primer name SPBC19G7.09.Rv
Rev primer SEQ AGAAAGCTGGGTAATAGATTTGTGCATCAATA
Amino acid length 568
Molecular weight 64.9
Isoelectric point (calc.) 9.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 23
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear envelope; cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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