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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC18E5.02c
Gene ID 35/D03
Gene synonyms/obsolete SPBC29A3.20c
Gene product putative serine palmitoyltransferase subunit
Entry clone Cloned
ORF length (unspliced) 1697
ORF length (spliced) 1530
Entry clone length 1697
No. of intron 3
Sequence status Finished
Sequence results 579T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC18E5.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTTACTCATATCCCTT
Rev primer name SPBC18E5.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAACTTGATTAATAACCTTA
Amino acid length 509
Molecular weight 57.4
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRTFIEFLLL/LTDVEKLEL/LLEEIVELCI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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