Gene name |
SPCC1450.10c |
Gene ID |
35/D02 |
Gene synonyms/obsolete |
|
Gene product |
iron only hydrogenase
large subunit; similar to human nuclear prelamin A recognition
factor |
Entry clone |
Cloned# |
ORF length (unspliced) |
1695 |
ORF length (spliced) |
1617 |
Entry clone length |
1695 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC1450.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAAAGTTGTCTGTAAA |
Rev primer name |
SPCC1450.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACCACTTATTGGCCAGCAAT |
Amino acid length |
538 |
Molecular weight |
60.4 |
Isoelectric point (calc.) |
6.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVEMFKFLRI |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |