Gene name |
SPAC23E2.01 |
Gene ID |
35/C10 |
Gene synonyms/obsolete |
fep1; gaf2 |
Gene product |
iron-sensing
transcriptional regulator; zinc finger protein; zf-GATA type;
involved in the regulation of the iron transporter gene
expression; expression constitutive irrespective of the
nitrogen source |
Entry clone |
Cloned# |
ORF length (unspliced) |
1695 |
ORF length (spliced) |
|
Entry clone length |
1695 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC23E2.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGCGAAACCTGCTCC |
Rev primer name |
SPAC23E2.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGTTTGAAGAGCTCCAGAT |
Amino acid length |
564 |
Molecular weight |
60.6 |
Isoelectric point (calc.) |
8.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus; nuclear
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |