Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23E2.01
Gene ID 35/C10
Gene synonyms/obsolete fep1; gaf2
Gene product iron-sensing transcriptional regulator; zinc finger protein; zf-GATA type; involved in the regulation of the iron transporter gene expression; expression constitutive irrespective of the nitrogen source
Entry clone Cloned#
ORF length (unspliced) 1695
ORF length (spliced)
Entry clone length 1695
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC23E2.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTGCGAAACCTGCTCC
Rev primer name SPAC23E2.01.Rv
Rev primer SEQ AGAAAGCTGGGTATGTTTGAAGAGCTCCAGAT
Amino acid length 564
Molecular weight 60.6
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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