Gene name |
SPAC24B11.07c |
Gene ID |
35/C01 |
Gene synonyms/obsolete |
|
Gene product |
ketopantoate
reductase; similar to Sp SPCC1902.02 (paralog) |
Entry clone |
Cloned |
ORF length (unspliced) |
1686 |
ORF length (spliced) |
|
Entry clone length |
1686 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
431A:G / 519A:G /
1122T:C / 1417T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC24B11.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTGACGCAGGAAAAAA |
Rev primer name |
SPAC24B11.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACGATTTCATGCGAGAAGTT |
Amino acid length |
561 |
Molecular weight |
62.4 |
Isoelectric point (calc.) |
8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDTLLPLEI/LISSIQHLPL |
Localization (YFP) |
periphery at cell tip
and site of septum formation; cytoplasmic microtubules |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>>cytosol;
periphery at cell tip) |
Microscope used for
observation |
DeltaVision |