| Gene name |
SPCC584.03c |
| Gene ID |
34/G05 |
| Gene synonyms/obsolete |
|
| Gene product |
RanGTP-binding
protein; similar to Sp SPCC584.03C (paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1656 |
| ORF length (spliced) |
|
| Entry clone length |
1656 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
375T:C / 1379T:A /
1638A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC584.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGAATTACTTAATGT |
| Rev primer name |
SPCC584.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACGGGTTTTTCTTTTCTTTT |
| Amino acid length |
551 |
| Molecular weight |
61.2 |
| Isoelectric point (calc.) |
5.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LREMKTLLL |
| Localization (YFP) |
nuclear envelope |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |