| Gene name |
SPBC3E7.08c |
| Gene ID |
34/A07 |
| Gene synonyms/obsolete |
rad13 |
| Gene product |
DNA repair nuclease;
involved in DNA repair; involved in nucleotide-excision
repair; XP-G family |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3339 |
| ORF length (spliced) |
|
| Entry clone length |
3339 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
22G:A / 2872T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3E7.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGGTTTCAGGTCTTTG |
| Rev primer name |
SPBC3E7.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTCTTCTTCTTTTGCTG |
| Amino acid length |
1112 |
| Molecular weight |
126.3 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LETLVNKRLAI/LSRVGPVLAL |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |