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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC21H7.05
Gene ID 33/E09
Gene synonyms/obsolete sfc6
Gene product RNA polymerase III transcription factor (TFIIIC subunit) (putative); WD repeat protein; AT hook (inferred)
Entry clone Cloned
ORF length (unspliced) 1881
ORF length (spliced) 1749
Entry clone length 1881
No. of intron 3
Sequence status Finished
Sequence results 1315C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC21H7.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGCCCTAAATCTAAGGA
Rev primer name SPBC21H7.05.Rv
Rev primer SEQ AGAAAGCTGGGTATCTTCTTTCAACGGCTGAG
Amino acid length 582
Molecular weight 66.2
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFEVKNLSI/LDDFPWLFL
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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