Gene name |
SPBC14F5.05c |
Gene ID |
32/E09 |
Gene synonyms/obsolete |
sam1 |
Gene product |
S-adenosylmethionine
synthetase; involved in methionine metabolism |
Entry clone |
Cloned# |
ORF length (unspliced) |
1149 |
ORF length (spliced) |
|
Entry clone length |
1149 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC14F5.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTCAAACTTTTTTGTT |
Rev primer name |
SPBC14F5.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAACTTAAGCTCCTTAGGT |
Amino acid length |
382 |
Molecular weight |
41.8 |
Isoelectric point (calc.) |
5.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |