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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1952.07
Gene ID 32/E08
Gene synonyms/obsolete rad1
Gene product involved in DNA repair, DNA damage checkpoint, DNA replication checkpoint, cell cycle checkpoint
Entry clone Cloned
ORF length (unspliced) 1141
ORF length (spliced) 972
Entry clone length 1141
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1952.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTCAAGCAGAAACAGT
Rev primer name SPAC1952.07.Rv
Rev primer SEQ AGAAAGCTGGGTAGCTATCCTCATCCTCGGTC
Amino acid length 323
Molecular weight 36.5
Isoelectric point (calc.) 4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDNNMGENLII
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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