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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23G3.09
Gene ID 32/E05
Gene synonyms/obsolete
Gene product RNA polymerase II general transcription factor TFIID component
Entry clone Cloned
ORF length (unspliced) 1098
ORF length (spliced)
Entry clone length 1098
No. of intron 0
Sequence status Finished
Sequence results 532A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23G3.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTTAAATAATCCTCC
Rev primer name SPAC23G3.01.Rv
Rev primer SEQ AGAAAGCTGGGTAATCTTTTAGCCTAATGTAA
Amino acid length 365
Molecular weight 40.5
Isoelectric point (calc.) 9.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 4 images) See all images

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