| Gene name |
SPBC543.07 |
| Gene ID |
32/E04 |
| Gene synonyms/obsolete |
skh1; pek1; mkk1 |
| Gene product |
serine/threonine
protein kinase; MAP kinase kinase (MAPKK); acts as a
phosphorylation-dependent molecular switch; mkh1 signaling
pathway component |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1092 |
| ORF length (spliced) |
|
| Entry clone length |
1092 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC543.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCAAAAAACCTGTCTT |
| Rev primer name |
SPBC543.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCAGACCAGACTTGACGA |
| Amino acid length |
363 |
| Molecular weight |
40.7 |
| Isoelectric point (calc.) |
8.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQKQLLRELKI |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |