| Gene name |
SPBC16D10.09 |
| Gene ID |
32/A11 |
| Gene synonyms/obsolete |
pcn1; pcn |
| Gene product |
PCNA; DNA polymerase
processivity factor activity; interacts with D cyclins;
overexpression results in cell cycle defects; involved in DNA
repair |
| Entry clone |
Cloned |
| ORF length (unspliced) |
894 |
| ORF length (spliced) |
783 |
| Entry clone length |
894 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC16D10.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTGAAGCTAGATTTCA |
| Rev primer name |
SPBC16D10.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCCTCATCCTCCTCACCA |
| Amino acid length |
260 |
| Molecular weight |
28.9 |
| Isoelectric point (calc.) |
4.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol;
nuclear dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |