Gene name |
SPBC16D10.09 |
Gene ID |
32/A11 |
Gene synonyms/obsolete |
pcn1; pcn |
Gene product |
PCNA; DNA polymerase
processivity factor activity; interacts with D cyclins;
overexpression results in cell cycle defects; involved in DNA
repair |
Entry clone |
Cloned |
ORF length (unspliced) |
894 |
ORF length (spliced) |
783 |
Entry clone length |
894 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16D10.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTGAAGCTAGATTTCA |
Rev primer name |
SPBC16D10.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCCTCATCCTCCTCACCA |
Amino acid length |
260 |
Molecular weight |
28.9 |
Isoelectric point (calc.) |
4.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol;
nuclear dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |