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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC119.02
Gene ID 32/A10
Gene synonyms/obsolete ubc4
Gene product ubiquitin conjugating enzyme; involved in mitotic transition, the protein ubiquitination of Cdc13p, ubiquitin chain elongation
Entry clone Cloned
ORF length (unspliced) 894
ORF length (spliced) 444
Entry clone length 894
No. of intron 2
Sequence status Finished
Sequence results 35A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC119.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTTTGAAAAGAATTAA
Rev primer name SPBC119.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGATTGCGTATTTTCTAGTC
Amino acid length 147
Molecular weight 16.4
Isoelectric point (calc.) 6.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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