| Gene name |
SPBC1709.18 |
| Gene ID |
32/A06 |
| Gene synonyms/obsolete |
tif452;
SPBC409.01 |
| Gene product |
translation initiation
factor (eIF4E subunit); eIF4G-binding |
| Entry clone |
Cloned |
| ORF length (unspliced) |
879 |
| ORF length (spliced) |
732 |
| Entry clone length |
879 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
206A:G / 463T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1709.18.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGATGCAGAAGACTC |
| Rev primer name |
SPBC1709.18.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGACTCATACGGGTTTTT |
| Amino acid length |
243 |
| Molecular weight |
27.6 |
| Isoelectric point (calc.) |
7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
Leica |