Gene name |
SPAC4D7.12c |
Gene ID |
32/A05 |
Gene synonyms/obsolete |
fet5 |
Gene product |
ATP-binding protein;
eukaryotic transcription factor |
Entry clone |
Cloned |
ORF length (unspliced) |
876 |
ORF length (spliced) |
831 |
Entry clone length |
876 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
46A:G / 301T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4D7.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTAAGGTGGCAGCTTT |
Rev primer name |
SPAC4D7.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCATCGTCTTCTAAATCA |
Amino acid length |
276 |
Molecular weight |
31.5 |
Isoelectric point (calc.) |
3.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |