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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC31A2.05c
Gene ID 31/B01
Gene synonyms/obsolete mis4
Gene product adherin; sister chromatid cohesion molecule; chromatin associated; involved in S phase and spindle kinetochore attachment
Entry clone Cloned
ORF length (unspliced) 5040
ORF length (spliced) 4752
Entry clone length 5040
No. of intron 7
Sequence status Finished
Sequence results 617A:T / 846T:C / 949A:G / 1012T:C / 1233A:C / 3071T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC31A2.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACACCTGAAACCTTTAA
Rev primer name SPAC31A2.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGACAACTGTAAGCTGTTCC
Amino acid length 1583
Molecular weight 180.2
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLQKVANVLNI/LLEDLLNMLSL/LNLKFFVSLII/LFDLSYLHL/LIMQILKPLKL
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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