| Gene name |
SPBC2F12.05c |
| Gene ID |
30/G09 |
| Gene synonyms/obsolete |
|
| Gene product |
involved in ergosterol
biosynthesis; oxysterol binding protein; ankyrin repeat
protein; pleckstrin homology domain; similar to Sp
SPAC23H4.01 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4304 |
| ORF length (spliced) |
3933 |
| Entry clone length |
4304 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
1214A:G /
2335A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC2F12.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACATCCGTCCCCTGA |
| Rev primer name |
SPBC2F12.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAAATATCATCAACATTA |
| Amino acid length |
1310 |
| Molecular weight |
148.4 |
| Isoelectric point (calc.) |
7.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
1245 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSDSLKQLQL/LHKTVLEMLDL |
| Localization (YFP) |
cytosol; cytoplasmic
dots, especially at site of septum formation by over
expression |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |