Gene name |
SPAC15E1.01 |
Gene ID |
30/F12 |
Gene synonyms/obsolete |
fat1;
SPAC15A10.16 |
Gene product |
actin binding protein;
involved in actin cytoskeletal organization |
Entry clone |
Cloned |
ORF length (unspliced) |
4158 |
ORF length (spliced) |
|
Entry clone length |
4158 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
2062T:C /
2884A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC15E1.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTAATAACGGCGATAA |
Rev primer name |
SPAC15E1.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTTAGGCTTGTCTCTTCA |
Amino acid length |
1385 |
Molecular weight |
154.3 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cell tip and site of
septum formation |
Comments for localization |
aggregates by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |