| Gene name |
SPAC17A5.14 |
| Gene ID |
30/F03 |
| Gene synonyms/obsolete |
exo2 |
| Gene product |
exonuclease II;
zf-C2H2 type; mutants are caffeine sensitive |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4075 |
| ORF length (spliced) |
3987 |
| Entry clone length |
4075 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC17A5.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAATTCCAAAGGTTGT |
| Rev primer name |
SPAC17A5.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTTAATCTGAAGTTTGCCC |
| Amino acid length |
1328 |
| Molecular weight |
152.5 |
| Isoelectric point (calc.) |
7.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDADLIMLGL/LPHLPGLHI/LRRLCNDLHL/LLNIHPLLL |
| Localization (YFP) |
cytosol; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>>cytosol) |
| Microscope used for
observation |
Leica |