Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17A5.14
Gene ID 30/F03
Gene synonyms/obsolete exo2
Gene product exonuclease II; zf-C2H2 type; mutants are caffeine sensitive
Entry clone Cloned
ORF length (unspliced) 4075
ORF length (spliced) 3987
Entry clone length 4075
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17A5.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGAATTCCAAAGGTTGT
Rev primer name SPAC17A5.14.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTAATCTGAAGTTTGCCC
Amino acid length 1328
Molecular weight 152.5
Isoelectric point (calc.) 7.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDADLIMLGL/LPHLPGLHI/LRRLCNDLHL/LLNIHPLLL
Localization (YFP) cytosol; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus>>cytosol)
Microscope used for observation Leica

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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