Gene name |
SPCC330.11 |
Gene ID |
30/E11 |
Gene synonyms/obsolete |
|
Gene product |
BTB domain; ankyrin
repeat protein; regulator of condensation |
Entry clone |
Cloned |
ORF length (unspliced) |
4044 |
ORF length (spliced) |
|
Entry clone length |
4044 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
409A:G / 1535A:G /
3670C:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC330.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTCATCTGCTTTTCGC |
Rev primer name |
SPCC330.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTGACTGCACGTGCTTTT |
Amino acid length |
1347 |
Molecular weight |
152.5 |
Isoelectric point (calc.) |
8.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
1308 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSSTIHPVLDL/LSKLANLLEL/LETMFEILSI/LPTLMDLSL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |