| Gene name |
SPAC1006.06 |
| Gene ID |
30/E10 |
| Gene synonyms/obsolete |
rgf2 |
| Gene product |
GDP-GTP exchange
protein for Rho1p; pleckstrin homology domain; CNH domain;
non-essential; overexpression causes defects in cell
separation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3576 |
| ORF length (spliced) |
|
| Entry clone length |
3576 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1006.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTCGCAATGGAGCTCA |
| Rev primer name |
SPAC1006.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGTGCAGGACCTGCTGCA |
| Amino acid length |
1158 |
| Molecular weight |
130.8 |
| Isoelectric point (calc.) |
9.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEPIFKLQL/LAEKILYELPL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |