| Gene name |
SPAC328.06 |
| Gene ID |
30/E06 |
| Gene synonyms/obsolete |
ubp2 |
| Gene product |
ubiquitin C-terminal
hydrolase activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3510 |
| ORF length (spliced) |
3426 |
| Entry clone length |
3510 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
192T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC328.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAACTGTAAAGAACTT |
| Rev primer name |
SPAC328.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGCAGAAGATTGTGTTCA |
| Amino acid length |
1141 |
| Molecular weight |
130.2 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFTTFTSLEL/LPIILQLQI/LMSSVDTLSI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |