Gene name |
SPCC1322.12c |
Gene ID |
30/A12 |
Gene synonyms/obsolete |
bub1 |
Gene product |
spindle assembly
checkpoint component; essential; mitotic centromere protein;
serine/threonine protein kinase |
Entry clone |
Cloned |
ORF length (unspliced) |
3135 |
ORF length (spliced) |
|
Entry clone length |
3135 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1823T:deletion |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1322.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCGATTGGCGGCTTAC |
Rev primer name |
SPCC1322.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAATTTTTCTTTTTTCGATG |
Amino acid length |
1044 |
Molecular weight |
118.3 |
Isoelectric point (calc.) |
6.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEEIQRLAL/LNDVRHLLI/LWHRLFDLLL |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |