Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCP25A2.02c
Gene ID 29/G03
Gene synonyms/obsolete rhp26
Gene product involved in nucleotide-excision repair; involved in DNA repair; involved in meiotic recombination; SNF2 family; DEAD/DEAH box helicase; helicase C-terminal domain; human Cockayne syndrome B homolog
Entry clone Cloned
ORF length (unspliced) 2922
ORF length (spliced)
Entry clone length 2922
No. of intron 0
Sequence status Finished
Sequence results 1901T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCP25A2.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTGTCAATGAAGATCT
Rev primer name SPCP25A2.02.Rv
Rev primer SEQ AGAAAGCTGGGTATCGTCTCTGTTTTAGTCGG
Amino acid length 973
Molecular weight 110.9
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKVIRALLTL
Localization (YFP) nucleus>>cytosol; nuclear dots and cytoplasmic dots by over expression
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus>>cytosol)
Microscope used for observation Confocal

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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