| Gene name |
SPAC8C9.06c |
| Gene ID |
29/D10 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; hypothetical protein; PPR domains; no apparent
Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2796 |
| ORF length (spliced) |
|
| Entry clone length |
2796 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
558T:C / 1416T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC8C9.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCAAGTCGTTTGCCTA |
| Rev primer name |
SPAC8C9.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGACCTTGGCTAATAAATTA |
| Amino acid length |
931 |
| Molecular weight |
107.9 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLLPFFTLDL/LGKIRRLGI/LNQLDQLLLL/LSRQFWDLIL |
| Localization (YFP) |
cytoplasmic dots |
| Comments for localization |
mitochondrion? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |