Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1683.04
Gene ID 29/A09
Gene synonyms/obsolete
Gene product beta-glucosidase activity; glycosyl hydrolase family 3; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 2499
ORF length (spliced)
Entry clone length 2499
No. of intron 0
Sequence status Finished
Sequence results 1128T:C / 1801T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1683.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATGGAACATGATGTTGA
Rev primer name SPBC1683.04.Rv
Rev primer SEQ AGAAAGCTGGGTATAAACCAGTCCAATGAATT
Amino acid length 832
Molecular weight 93.1
Isoelectric point (calc.) 5.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKKAIDAGLDL/LLFIDDVLLI
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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