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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F7.11c
Gene ID 29/A06
Gene synonyms/obsolete
Gene product transcriptional regulator; zinc finger protein; zf-fungal Zn(2)-Cys(6) binuclear cluster domain; similar to Sp SPAC139.03 and SPBC16G5.16 and SPAC3C7.04 (paralogs)
Entry clone Cloned
ORF length (unspliced) 2496
ORF length (spliced) 2349
Entry clone length 2496
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1F7.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCGCGTCTGGCAAAAA
Rev primer name SPAC1F7.11.Rv
Rev primer SEQ AGAAAGCTGGGTACTTCGGAATACCCATTTCG
Amino acid length 782
Molecular weight 89.3
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: nucleus>cytosol
Microscope used for observation Leica

Image information
YFP 1 images) See all images
LMB 2 images) See all images

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