| Gene name |
SPAC1F7.11c |
| Gene ID |
29/A06 |
| Gene synonyms/obsolete |
|
| Gene product |
transcriptional
regulator; zinc finger protein; zf-fungal Zn(2)-Cys(6)
binuclear cluster domain; similar to Sp SPAC139.03 and
SPBC16G5.16 and SPAC3C7.04 (paralogs) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2496 |
| ORF length (spliced) |
2349 |
| Entry clone length |
2496 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1F7.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCGCGTCTGGCAAAAA |
| Rev primer name |
SPAC1F7.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTCGGAATACCCATTTCG |
| Amino acid length |
782 |
| Molecular weight |
89.3 |
| Isoelectric point (calc.) |
6.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
Leica |