| Gene name |
SPAC16A10.01 |
| Gene ID |
29/A03 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; hypothetical protein; Sc YJL108C is regulated by
pheromone |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2493 |
| ORF length (spliced) |
|
| Entry clone length |
2493 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC16A10.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAGTCCAGCTTAAGTT |
| Rev primer name |
SPAC16A10.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGCATCATGTTTTTTCGT |
| Amino acid length |
830 |
| Molecular weight |
91.6 |
| Isoelectric point (calc.) |
8.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
9 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIGFVLGLLL/LYAVIFSLFL/LLIPIFTLSL |
| Localization (YFP) |
cytoplasmic dots at
cell tip and site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |