Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC14C4.11
Gene ID 28/F08
Gene synonyms/obsolete
Gene product polyphosphate synthetase; similar to Sp SPCC1322.14C (paralog)
Entry clone Cloned
ORF length (unspliced) 2412
ORF length (spliced) 2205
Entry clone length 2412
No. of intron 1
Sequence status Finished
Sequence results 638A:T / 2342T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC14C4.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGTTTCAGTGACAGTAT
Rev primer name SPAC14C4.11.Rv
Rev primer SEQ AGAAAGCTGGGTAAGTAAACAATTTAACAAGC
Amino acid length 734
Molecular weight 85.4
Isoelectric point (calc.) 7.1
Signal SEQ
No. of transmembrane domain 3
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDPISTCLYL
Localization (YFP) ambiguous structure
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.