Gene name |
SPAC14C4.11 |
Gene ID |
28/F08 |
Gene synonyms/obsolete |
|
Gene product |
polyphosphate
synthetase; similar to Sp SPCC1322.14C (paralog) |
Entry clone |
Cloned |
ORF length (unspliced) |
2412 |
ORF length (spliced) |
2205 |
Entry clone length |
2412 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
638A:T / 2342T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC14C4.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGTTTCAGTGACAGTAT |
Rev primer name |
SPAC14C4.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTAAACAATTTAACAAGC |
Amino acid length |
734 |
Molecular weight |
85.4 |
Isoelectric point (calc.) |
7.1 |
Signal SEQ |
|
No. of transmembrane domain |
3 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDPISTCLYL |
Localization (YFP) |
ambiguous
structure |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |