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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3A12.15
Gene ID 28/B07
Gene synonyms/obsolete vps53
Gene product involved in intracellular protein transport; involved in vacuolar sorting; GARP complex; involved in protein sorting in the late Golgi; involved in retrograde transport from the prevacuole; involved in docking at the trans-Golgi network; similar to S. cerevisiae YJL029C
Entry clone Cloned
ORF length (unspliced) 2271
ORF length (spliced)
Entry clone length 2271
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3A12.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGAATGGAAATGATAA
Rev primer name SPAC3A12.15.Rv
Rev primer SEQ AGAAAGCTGGGTAAACGTGCTTTTTCTTTGAA
Amino acid length 756
Molecular weight 87.5
Isoelectric point (calc.) 7.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVGLKNLFI
Localization (YFP) cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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