Gene name |
SPAC3A12.15 |
Gene ID |
28/B07 |
Gene synonyms/obsolete |
vps53 |
Gene product |
involved in
intracellular protein transport; involved in vacuolar sorting;
GARP complex; involved in protein sorting in the late Golgi;
involved in retrograde transport from the prevacuole; involved
in docking at the trans-Golgi network; similar to S.
cerevisiae YJL029C |
Entry clone |
Cloned |
ORF length (unspliced) |
2271 |
ORF length (spliced) |
|
Entry clone length |
2271 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC3A12.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGAATGGAAATGATAA |
Rev primer name |
SPAC3A12.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACGTGCTTTTTCTTTGAA |
Amino acid length |
756 |
Molecular weight |
87.5 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVGLKNLFI |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |