| Gene name |
SPBC887.04c |
| Gene ID |
28/B06 |
| Gene synonyms/obsolete |
|
| Gene product |
WD repeat protein;
PLAP family; involved in ubiquitin-dependent proteolysis;
interacts physically with SPAC1565.08; involved in DNA repair;
RAD6 epistasis group; similar to mammalian phospholipase
a-2-activating protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2268 |
| ORF length (spliced) |
2142 |
| Entry clone length |
2268 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
2069T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC887.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACCTCTTATGAACTTTC |
| Rev primer name |
SPBC887.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAGAGTGAAAGGATTTGC |
| Amino acid length |
713 |
| Molecular weight |
78.6 |
| Isoelectric point (calc.) |
4.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDALRLLAI/LAMNLSILLI |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |