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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC6F12.17
Gene ID 28/A11
Gene synonyms/obsolete
Gene product HAT repeat protein (SMART); involved in polyadenylation; involved in 3' end maturation
Entry clone Cloned
ORF length (unspliced) 2245
ORF length (spliced) 2202
Entry clone length 2245
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC6F12.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATTCTGATGACAATGT
Rev primer name SPAC6F12.17.Rv
Rev primer SEQ AGAAAGCTGGGTAATTAAATCTGGCACGCTTC
Amino acid length 733
Molecular weight 83.9
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSVDLWTLYL/LDGFSYFLSI
Localization (YFP) nuclear dots; nucleus
Comments for localization
Effect of LMB on protein localization changed to: nucleus>cytosol
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images
LMB 4 images) See all images

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