Gene name |
SPBC428.07 |
Gene ID |
27/G05 |
Gene synonyms/obsolete |
meu6 |
Gene product |
lysine-rich protein;
predicted coiled-coil region; meiotic expression upregulated;
NLS (bipartite); pleckstrin homolgy domain; no orthologs |
Entry clone |
Cloned |
ORF length (unspliced) |
2083 |
ORF length (spliced) |
1956 |
Entry clone length |
2083 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC428.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTATGAGGGCCGTGA |
Rev primer name |
SPBC428.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCGTTAACGGTAATGGAC |
Amino acid length |
651 |
Molecular weight |
71.1 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus;
periphery |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|