Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC15A10.01
Gene ID 27/G04
Gene synonyms/obsolete SPAC8C9.18
Gene product ABC family; membrane transporter
Entry clone Cloned
ORF length (unspliced) 2082
ORF length (spliced)
Entry clone length 2082
No. of intron 0
Sequence status Finished
Sequence results 44A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC15A10.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTAGAACGATGTCCCTG
Rev primer name SPAC15A10.01.Rv
Rev primer SEQ AGAAAGCTGGGTATGCATCTCCGGATTTATTC
Amino acid length 693
Molecular weight 77
Isoelectric point (calc.) 10
Signal SEQ
No. of transmembrane domain 5
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKVRVVSALAL/LHIIPITLEI
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.