Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1071.01c
Gene ID 27/D12
Gene synonyms/obsolete SPAC4H3.15c
Gene product involved in cleavage and polyadenylation
Entry clone Cloned
ORF length (unspliced) 2013
ORF length (spliced)
Entry clone length 2013
No. of intron 0
Sequence status Finished
Sequence results 1259A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1071.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGTTTCGGAGACAGA
Rev primer name SPAC1071.01.Rv
Rev primer SEQ AGAAAGCTGGGTACGTACAGGTTAGTCGAAAC
Amino acid length 670
Molecular weight 76.2
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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