| Gene name |
SPAC25B8.11 |
| Gene ID |
27/D11 |
| Gene synonyms/obsolete |
|
| Gene product |
involved in
transcriptional regulation; zinc finger protein; possible
activator for allantoin, 4-aminobutyric acid (GABA), and urea
catabolic genes |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2011 |
| ORF length (spliced) |
1965 |
| Entry clone length |
2011 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
105T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC25B8.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAATTTGATTTTAAC |
| Rev primer name |
SPAC25B8.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCGGACTTGGGAAACGTC |
| Amino acid length |
654 |
| Molecular weight |
74.7 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVSLLIRLHL |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |