Gene name |
SPCC4G3.09c |
Gene ID |
26/F05 |
Gene synonyms/obsolete |
|
Gene product |
TBC domain protein;
GTPase activating protein of Rab-like GTPase |
Entry clone |
Cloned |
ORF length (unspliced) |
1908 |
ORF length (spliced) |
|
Entry clone length |
1908 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
7G:A / 18A:C / 135T:C
/ 619G:A / 1170T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC4G3.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGGCATCACTGAACA |
Rev primer name |
SPCC4G3.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTAGACTTCTTCAAGTGA |
Amino acid length |
635 |
Molecular weight |
72.7 |
Isoelectric point (calc.) |
6.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |