Gene name |
SPAC2F7.11 |
Gene ID |
25/F01 |
Gene synonyms/obsolete |
nrd1 |
Gene product |
negative regulator of
differentiation; rrm RNA recognition motif; RNA-binding
protein; block the onset of sexual development by repressing
the Ste11p |
Entry clone |
Cloned |
ORF length (unspliced) |
1590 |
ORF length (spliced) |
|
Entry clone length |
1590 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC2F7.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTCTAGCAGTCCCTC |
Rev primer name |
SPAC2F7.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAATATTGGAGTTTGTTGGC |
Amino acid length |
529 |
Molecular weight |
57.7 |
Isoelectric point (calc.) |
8.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
264 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |