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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2F7.11
Gene ID 25/F01
Gene synonyms/obsolete nrd1
Gene product negative regulator of differentiation; rrm RNA recognition motif; RNA-binding protein; block the onset of sexual development by repressing the Ste11p
Entry clone Cloned
ORF length (unspliced) 1590
ORF length (spliced)
Entry clone length 1590
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2F7.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTCTAGCAGTCCCTC
Rev primer name SPAC2F7.11.Rv
Rev primer SEQ AGAAAGCTGGGTAAATATTGGAGTTTGTTGGC
Amino acid length 529
Molecular weight 57.7
Isoelectric point (calc.) 8.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 264
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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