| Gene name |
SPCC794.07 |
| Gene ID |
25/E12 |
| Gene synonyms/obsolete |
|
| Gene product |
dihydrolipoamide
S-acetyltransferase activity; involved in acetyl-CoA
biosynthesis from pyruvate; localization pyruvate
dehydrogenase complex (sensu Eukarya) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1589 |
| ORF length (spliced) |
1452 |
| Entry clone length |
1589 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC794.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTATCAGCAAATATGCT |
| Rev primer name |
SPCC794.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGCATAATCTCCAATGGA |
| Amino acid length |
483 |
| Molecular weight |
52 |
| Isoelectric point (calc.) |
8.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKKILENPLEI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |