Gene name |
SPCC1529.01 |
Gene ID |
25/B08 |
Gene synonyms/obsolete |
SPCC794.14 |
Gene product |
MFS multidrug efflux
transporter |
Entry clone |
Cloned |
ORF length (unspliced) |
1552 |
ORF length (spliced) |
1389 |
Entry clone length |
1552 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1529.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATCTAGAAAAGAAACC |
Rev primer name |
SPCC1529.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAAACATACATAAATGCC |
Amino acid length |
462 |
Molecular weight |
51.8 |
Isoelectric point (calc.) |
9.2 |
Signal SEQ |
|
No. of transmembrane domain |
9 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEHIPRLKL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |