Gene name |
SPAC17A2.13c |
Gene ID |
24/G12 |
Gene synonyms/obsolete |
rad25; cps6 |
Gene product |
DNA damage checkpoint
protein Rad25 |
Entry clone |
Cloned |
ORF length (unspliced) |
1530 |
ORF length (spliced) |
813 |
Entry clone length |
1530 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC17A2.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTAATTCTCGTGAAAA |
Rev primer name |
SPAC17A2.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCTTTAACAGTGTCAGTC |
Amino acid length |
270 |
Molecular weight |
30.3 |
Isoelectric point (calc.) |
4.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus;
periphery at cell tip and site of septum formation |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |