Gene name |
SPAC2C4.06c |
Gene ID |
24/D07 |
Gene synonyms/obsolete |
|
Gene product |
NOL1/NOP2/sun family;
methyltransferase |
Entry clone |
Cloned# |
ORF length (unspliced) |
1505 |
ORF length (spliced) |
1368 |
Entry clone length |
1505 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC2C4.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTTTTATAATCATGC |
Rev primer name |
SPAC2C4.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCTTCACCTTGTATGCGA |
Amino acid length |
455 |
Molecular weight |
51.2 |
Isoelectric point (calc.) |
9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
435/433/436/438/437/434 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |