Gene name |
SPBC800.13 |
Gene ID |
23/B12 |
Gene synonyms/obsolete |
|
Gene product |
Histone-fold/TFIID-TAF/NF-Y (Interpro); DNA-binding
protein; involved in transcriptional regulation; no apparetn
Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1440 |
ORF length (spliced) |
|
Entry clone length |
1440 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC800.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTCTCACAATTATGA |
Rev primer name |
SPBC800.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGTTCTCTTAATTGCCGGA |
Amino acid length |
479 |
Molecular weight |
54.3 |
Isoelectric point (calc.) |
10.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
SPB; nucleus |
Comments for localization |
faint signal of
mitochondrion |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |