| Gene name |
SPBC800.13 |
| Gene ID |
23/B12 |
| Gene synonyms/obsolete |
|
| Gene product |
Histone-fold/TFIID-TAF/NF-Y (Interpro); DNA-binding
protein; involved in transcriptional regulation; no apparetn
Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1440 |
| ORF length (spliced) |
|
| Entry clone length |
1440 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC800.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTCTCACAATTATGA |
| Rev primer name |
SPBC800.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGGTTCTCTTAATTGCCGGA |
| Amino acid length |
479 |
| Molecular weight |
54.3 |
| Isoelectric point (calc.) |
10.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB; nucleus |
| Comments for localization |
faint signal of
mitochondrion |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |